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keratinocyte growth medium 2  (PromoCell)


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    PromoCell keratinocyte growth medium 2
    Keratinocyte Growth Medium 2, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 384 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/keratinocyte+growth+medium+2/Keratinocyte+Growth+Medium+2/pm42159017-45-4-14
    Average 96 stars, based on 384 article reviews
    keratinocyte growth medium 2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Altered Carnitine Homeostasis Modulates Hyperosmolarity-Induced Mitochondrial Dysfunction and Lipotoxicity in Human Corneal Epithelial Cells.
    Article Snippet: .. Human telomerase-immortalized corneal epithelial (hTCEpi) cells22 were cultured in complete Keratinocyte Growth Medium-2 (KGM-2; C-20111; PromoCell, Heidelberg, Germany), as previously described.10 Prior to treatment, cells were maintained in basal KGM-2 medium without supplements. ..

    Article Title: A Multiaxial Bioreactor Integrated with an Environmental Monitoring System for Tissue-Engineered Skin Grafts.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reativ The keratinocyte cell line, the Ker-CT (ATCC, Manassas, VA, USA) was purchased from ATCC, and cells were cultured in Keratinocyte Growth Medium 2 (C-20011, Heidelberg, Germany, PromoCell). .. At 70% confluency cells were washed with DPBS, followed by trypsinization using 0.25% Trypsin/0.53 mm EDTA (Thermo Fisher Scientific).

    Article Title: Keratinocyte Priming by Staphylococcus aureus Reduces HSV-1 Susceptibility.
    Article Snippet: .. Cells were cultured in keratinocyte growth medium 2 (Keratinocyte Basal Medium 2 plus supplements, PromoCell) without antibiotics at 37°C in a 5% CO2 atmosphere. ..

    Article Title: Radiometric and spectral characterization of multipanel planar light-emitting devices: a generalizable irradiance mapping approach for dose estimation
    Article Snippet: Primary normal human epidermal keratinocytes, isolated from the epidermis of juvenile foreskin or adult skin from pooled donors, were purchased from PromoCell (Heidelberg, Germany; Cat. No. C-12006). .. Cells were maintained in Keratinocyte Growth Medium 2 (KGM2; PromoCell), a serum-free cell culture medium for keratinocytes from the epidermis supplemented with 4 mL/L bovine pituitary extract, 0.125 ng/mL epidermal growth factor (recombinant human), 5 μg / mL insulin (recombinant human), 0.33 μg / mL hydrocortisone, 0.39 μg / mL epinephrine, 10 μg / mL transferrin (recombinant human), 0.06 mM CaCl 2 , 50 I.U./mL penicillin, and 50 μg / mL streptomycin. .. Cells were incubated at 37°C in the dark, in the presence of 5% CO 2 (Sanyo CO 2 incubator, Osaka, Japan) until the confluence reached 90%.

    Concentration Assay:

    Article Title: Inactivation of Focal Adhesion Kinase <scp>FAK</scp> Rapidly Abrogates Keratinocyte Entry in Mitosis via Rho‐Associated Kinase, Resulting in Squamous Differentiation
    Article Snippet: .. For this case, after confluence, keratinocytes were transferred to low calcium concentration (< 0.1 mM) in Keratinocyte growth medium 2 (C- 20111; Promocell) or Defined Keratinocyte- SFM (serum free; < 0.1 mM Ca+2; 10 744 019; Gibco), in accord with manufacturer's instructions, and lentiviral infections were performed as previously described [39]. ..

    Plasmid Preparation:

    Article Title: Cutaneous human papillomavirus E6 impairs the cGAS-STING pathway
    Article Snippet: .. Cells were grown in Keratinocyte Growth Medium 2 (PromoCell) supplemented with calcium chloride (PromoCell), SupplementMix (PromoCell), and penicillin-streptomycin (Caisson). hTERT HFKs expressed empty vector control (LXSN) and HA-tagged β-HPV8 E6 driven by the promoter in the LXSN vector. ..

    Control:

    Article Title: Cutaneous human papillomavirus E6 impairs the cGAS-STING pathway
    Article Snippet: .. Cells were grown in Keratinocyte Growth Medium 2 (PromoCell) supplemented with calcium chloride (PromoCell), SupplementMix (PromoCell), and penicillin-streptomycin (Caisson). hTERT HFKs expressed empty vector control (LXSN) and HA-tagged β-HPV8 E6 driven by the promoter in the LXSN vector. ..

    Recombinant:

    Article Title: Radiometric and spectral characterization of multipanel planar light-emitting devices: a generalizable irradiance mapping approach for dose estimation
    Article Snippet: Primary normal human epidermal keratinocytes, isolated from the epidermis of juvenile foreskin or adult skin from pooled donors, were purchased from PromoCell (Heidelberg, Germany; Cat. No. C-12006). .. Cells were maintained in Keratinocyte Growth Medium 2 (KGM2; PromoCell), a serum-free cell culture medium for keratinocytes from the epidermis supplemented with 4 mL/L bovine pituitary extract, 0.125 ng/mL epidermal growth factor (recombinant human), 5 μg / mL insulin (recombinant human), 0.33 μg / mL hydrocortisone, 0.39 μg / mL epinephrine, 10 μg / mL transferrin (recombinant human), 0.06 mM CaCl 2 , 50 I.U./mL penicillin, and 50 μg / mL streptomycin. .. Cells were incubated at 37°C in the dark, in the presence of 5% CO 2 (Sanyo CO 2 incubator, Osaka, Japan) until the confluence reached 90%.



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    PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in KSFM medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).

    Journal: PLOS One

    Article Title: Fatty acid-binding protein 5 (FABP5) modulates limbal epithelial cell homeostasis by regulating the expression of key genes under both normal and inflammatory conditions , in vitro

    doi: 10.1371/journal.pone.0347228

    Figure Lengend Snippet: PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in KSFM medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).

    Article Snippet: After centrifugation at 200g for 5 minutes, cells were cultured in Keratinocyte growth medium (KSFM) (Cat. Nr. C-20111, Promocell, Heidelberg, Germany), at 37 oC, 5% CO 2 .

    Techniques: Expressing, Transfection, Control, Western Blot, Knockdown